The Lammerding Lab

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What squishing cells can tell us about human disease – studying the interplay between cellular structure, mechanics, and function.

Cells in the human body reside in a physically stressful and demanding environment, being continuously exposed to large forces and deformations. Examples include not only contracting skeletal and cardiac muscle cells, bone and cartilage, but also immune cells, stem cells, and metastatic cancer cells that move through tight tissue spaces to reach distant sites in the body. It is now well established that cells respond to such physical challenges with characteristic changes in cellular organization, signaling, and function through a process referred to as ‘mechanotransduction’. The molecular mechanisms by which cells sense their physical environment and translate mechanical stimuli into biochemical signals remains incompletely understood. Nonetheless, it has become abundantly clear that defects in cellular mechanics or mechanotransduction signaling—arising from mutations or altered gene expression—can disturb cellular function and contribute to a large number of human diseases, ranging from muscular dystrophy to heart disease and cancer.

In the Lammerding laboratory, we investigate this intricate interplay between cellular structure, mechanics and function through an interdisciplinary research approach that combines engineering principles, microfabrication, and cell and molecular biology techniques, as well as the development and application of novel experimental assays. Our team is composed of students and postdoctoral researchers with a broad range of backgrounds, including (biomedical) engineering, biology, chemistry and other life sciences, who explore creative solutions to relevant biological and clinical problems. Our research is focused on the nuclear envelope and its interface with the surrounding cytoskeleton, as mutations in nuclear envelope proteins are responsible for more than 10 human diseases, such as muscular dystrophy, dilated cardiomyopathy, and premature aging (Hutchison-Gilford progeria syndrome). Furthermore, abnormal expression of nuclear envelope proteins expression has recently been identified in numerous cancers, often correlating with negative clinical outcomes. Work conducted in the Lammerding lab has already shown that mutations or loss of nuclear envelope proteins such as lamins render cells more sensitive to mechanical stress and also impair mechanotransduction signaling, providing a potential mechanism for the progressive muscle defects in many nuclear envelopathies. Furthermore, we have demonstrated that the deformability of the nucleus, the largest and most rigid cell organelle, constitutes a rate-limiting factor in the ability of cells to migrate through tight spaces, stimulating increased interest in understanding how highly motile cells such as neutrophils and metastatic cancer cells overcome this challenge.

Please check our Research Section for more details.

Postdoc position available! Click here for more details.

Selected Publications

Earle AJ, Kirby TJ, Fedorchak GR, Isermann P, Patel J, Iruvanti S, Moore SA, Bonne G, Wallrath LL, Lammerding J. Mutant lamins cause nuclear envelope rupture and DNA damage in skeletal muscle cells. Nature Materials. 2020. 19: 464-473

Davidson PM, Fedorchak GR, Mondesert-Deveraux S, Bell ES, Isermann P, Aubry D, Allena R, Lammerding J. High-throughput microfluidic micropipette aspiration device to probe time-scale dependent nuclear mechanics in intact cells. Lab Chip. 2019. 19: 3652-3663

Kirby TJ. Lammerding J. Emerging views of the nucleus as a cellular mechanosensor. Nat Cell Biol. 2018. 20(4): 373-381

Bakhoum SF, Ngo B, Bakhoum AL, Cavallo JA, Murphy CJ, Ly P, Shah P, Sriram RK, Watkins TBK, Taunk NK, Duran M, Pauli C, Shaw C, Chadalavada K, Rajasekhar VK, Genovese G, Venkatesan S, Birkbak NJ, McGranahan N, Lundquist M, LaPlant Q, Healey JH, Elemento O, Chung CH, Lee NY, Imielenski M, Nanjangud G, Pe’er D, Cleveland D, Powell SN, Lammerding J, Swanton C, Cantley LC. Chromosomal instability promotes metastasis through a cytosolic DNA response. Nature. 2018. 553(7689): 467-472

Denais CM, Gilbert RM, Isermann P, McGregor AL, te Lindert M, Weigelin B, Davidson PM, Friedl P, Wolf K, Lammerding J. Nuclear envelope rupture and repair during cancer cell migrationScience. 2016. 352(6283): 353-358.

Davidson PM, Sliz J, Isermann P, Denais C, Lammerding J. Design of a microfluidic device to quantify intra-nuclear deformation during cell migration through confining environments. Integr Biol (Camb). 2015. 30(7): 1534-46.

Ho CY, Jaalouk DE, Vartiainen MK, Lammerding J. Lamin A/C and emerin regulate MKL1/SRF activity by modulating actin dynamics. Nature 2013. 497(7450):507-11.

Isermann P, Lammerding J. Nuclear Mechanics and Mechanotransduction in Health and Disease. Curr Biol. 2013. 23(24): R1113-R1121.

Zwerger M, Jaalouk DE, Lombardi ML, Isermann P, Mauermann M, Dialynas G, Herrmann H, Wallrath LL, Lammerding J. Myopathic lamin mutations impair nuclear stability in cells and tissue and disrupt nucleo-cytoskeletal coupling. Hum Mol Gen. 2013. 22(12):2335-49

Jan Lammerding, PhD

Growing up in Germany, Jan Lammerding initially followed a traditionally engineering trajectory. Taking apart everything from cameras and clocks to radios as a kid, he went on to study mechanical engineering at the Rheinisch Westfälische Technische Universität (University of Technology) Aachen, in Aachen, Germany. Supported by a fellowship from the Deutscher Akademischer Austausch Dienst (DAAD), he spent a year at the Thayer School of Engineering at Dartmouth College, where he earned a Bachelor of Engineering degree. The interdisciplinary environment at Thayer School reinforced his longstanding interest in combining engineering with biology. After receiving his Diplom Ingenieur degree in Germany, he completed a PhD in the newly founded Biological Engineering program at the Massachusetts Institute of Technology (MIT), designing new techniques to study the biophysical properties of cells under the supervision of Drs. Roger Kamm and Richard Lee.

Following a brief post-doctoral training in Dr. Richard Lee’s laboratory at Harvard Medical School/Brigham and Women’s Hospital, Jan Lammerding was appointed as Instructor and subsequently Assistant Professor at Harvard Medical School/Brigham and Women’s Hospital. During that time, he also served as Lecturer in the Department of Biological Engineering at MIT, teaching a class in molecular, cell, and tissue biomechanics in the new Biological Engineering major. In 2011, Jan Lammerding was recruited to the Department of Biomedical Engineering and the Weill Institute for Cell and Molecular Biology at Cornell University, where he is now an Associate Professor in Biomedical Engineering (BMW) and serves as the Director of Graduate Studies for the BME PhD program.

Weill Institute for Cell & Molecular Biology
Department of Biomedical Engineering
Cornell University
235 Weill Hall
Ithaca, NY 14853-7202
(607)255-1700 (office)
(607)255-1980 (lab)
(607)255-2472 (fax)


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